Part:BBa_K4765127

ribozyme connected: H. ex mtSSB + AnAFP + SAHS 33020

Introduction

Similar to BBa_K4765126, we changed the order of H. ex mtSSB, SAHS 33020 and AnAFP.

Characterization

Get details in BBa_K4765126.

Agarose gel electrophoresis

Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures. From left lane(1) to right lane(3) indicate the successful construction of H. ex mtSSB, H. ex mtSSB + AnAFP, and H. ex mtSSB + AnAFP + SAHS 33020.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 247
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1573
  Illegal BsaI.rc site found at 515
  Illegal BsaI.rc site found at 596
  Illegal SapI site found at 409
  Illegal SapI.rc site found at 1214